Abstract
In order to screen the candidate genes of Fenneropenaeus chinensis related to low-temperature tolerance, this research takes juvenile prawns of F. chinensis (P40) in low temperature stress group (4°C) and normal temperature group (18°C) as experimental materials. The results showed that a total of 127,939 Unigenes with average length of 1,190 bp were obtained by assembly, of which 46% were annotated in the Nr database. A total of 1,698 differentially expressed genes were screened by differential gene expression analysis, of which 920 genes showed up-regulated expression and 778 genes showed down-regulated expression. Both GO and KEGG enrichment analysis revealed that differentially expressed genes were enriched in spliceosomes, ribosomes, bile secretion, ABC transport pathways, and cellular nitrogen compound synthesis. A further in-depth analysis obtained 8 genes that may be associated with low-temperature traits of F. chinensis. Five of them displayed up-regulated expression, including ATP-binding cassette protein C, acid ceramidase, glutathione transferase, C-type lectin and heat shock protein HSP70. The remaining three genes, γ-butyl betaine hydroxylase, β-hexosaminidase A and long chain fatty acid-CoA ligase displayed down-regulated expression. Eight differentially expressed genes were randomly selected and the real time RT-PCR verification showed that their expression levels were consistent with the sequencing results, demonstrating the accuracy of the sequencing results. The results of this study provide basic data for revealing the molecular mechanisms of F. chinensis in response to low temperature stress and the molecular assisted breeding of F. chinensis in low temperature.
Highlights
Huang et al [6] used RNA-seq technology to analyze the transcriptome sequences of Litopenaeus vannamei in low temperature (13 ̊C) and normal temperature (28 ̊C) and obtained 72 differentially expressed genes
In order to explore the molecular mechanisms of F. chinensis low temperature tolerance, differentially expressed genes under low temperature stress and low-temperature-related metabolic pathways were screened in the present study by comparing transcriptome information between 40-day prawns in normal temperature group (18 ̊C) and low temperature groups (4 ̊C)
A total of 138 families of 40-day juvenile prawns belongs to G12 of new F. chinensis variety-‘Huanghai No 2’ that produced in 2017 were selected as the experimental subjects with average body weight 0.150 ± 0.088g
Summary
Huang et al [6] used RNA-seq technology to analyze the transcriptome sequences of Litopenaeus vannamei in low temperature (13 ̊C) and normal temperature (28 ̊C) and obtained 72 differentially expressed genes. In order to explore the molecular mechanisms of F. chinensis low temperature tolerance, differentially expressed genes under low temperature stress and low-temperature-related metabolic pathways were screened in the present study by comparing transcriptome information between 40-day prawns in normal temperature group (18 ̊C) and low temperature groups (4 ̊C).
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