Screening of supports for immobilization of commercial porcine pancreatic lipase

Robison Scherer,Sibele Pergher,J Vladimir Oliveira,Débora De Oliveira

doi:10.1590/s1516-14392011005000079

Abstract

The aim of this work is to report the performance of different supports for the immobilization of commercial porcine pancreatic lipase. The immobilization tests were carried out in several types of Accurel, activated alumina, kaolin, montmorillonite, ion exchange resins and zeolites. The characterization of the supports showed differences in terms of specific area and morphology. The characteristics of the supports influenced the amount of enzyme adsorbed, yield of immobilization and esterification activity of the resulting immobilized catalyst. The clays KSF and natural and pillared montmorillonites presented potential for use as support for lipase immobilization in terms of yield and esterification activity. Yields of immobilization of 76.32 and 52.01% were achieved for clays KSF and natural montmorillonite, respectively. Esterification activities of 754.03, 595.51, 591.88 and 515.71 U.g-1 were obtained for lipases immobilized in Accurel MP-100, Amberlite XAD-2, mordenite and pillared montmorillonite, respectively.

Highlights

Lipases catalyzing lipid modifications have attracted considerable attention over the last years
Based on the above mentioned aspects and the fact that no systematic work was found in the open literature about the immobilization of porcine pancreatic lipase, the aim of this work was to perform the screening of suitable supports for immobilization of this commercial lipase
The specific areas and X-ray diffraction were determined for each support used for lipase immobilization

Summary

Introduction

Lipases catalyzing lipid modifications have attracted considerable attention over the last years. The attractive aspects of this catalyst over chemical methods include the high specificity of some lipases, the mild conditions required for reactions to take place, thereby requiring minimal energy inputs, reduced levels of by-products generated during the reaction and more efficient conversion of thermo sensitive substrates[1,2,3]. These catalysts play an important role in biotechnology, in food and oil processing and in the preparation of chiral intermediates. The main advantages of immobilized enzymes are related to their higher stability and easy separation from the reaction medium[8]

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Methods

Conclusion