Screening of Serum Protein Markers for Avascular Osteonecrosis of Femoral Head Differentially Expressed after Treatment with Yuanshi Shengmai Chenggu Tablets.

Peng Deng,Jie Li,Yirong Zeng,Jianchun Zeng,Wenjun Feng,Jinlun Chen

doi:10.1155/2018/5692735

Abstract

Avascular necrosis of the femoral head (ANFH) is an a frequently occurring orthopaedic disease with high morbidity. Traditional Chinese Medicine (TCM) Yuanshi Shengmai Chenggu Tablet is a valid prescription for treating steroid-induced femoral head necrosis. However, there are rare investigations about the serum protein marker expression after the acting of drugs on hormone and TCM. In the present study, we aimed to systematically discover and validate the serum biomarkers expression difference in patients with steroid-induced avascular necrosis of femoral head (SANFH) after taking Yuanshi Shengmai Chenggu Tablets (SANFH-TCM), so as to reveal the action mechanism of TCM from the molecular level by using isobaric tags for relative and absolute quantification (iTRAQ) with multiple reaction monitoring quantification. Significant differences in fibrinogen alpha, fibrinogen beta, fibrinogen gamma, fibronectin, C-reactive protein, apolipoprotein A, apolipoprotein D, and apolipoprotein E were found among SANFH, SANFH-TCM, and healthy controls. Therefore, our study proposes potential biomarkers for SANFH diagnosis and for the prognosis of femoral head necrosis after Traditional Chinese Medicine treatment.

Highlights

Avascular necrosis of the femoral head (ANFH) is a clinical common orthopaedic disease [1,2,3] with very high morbidity
Secondary osteonecrosis of the femoral head could divide into traumatic and nontraumatic osteonecrosis of femoral head [6,7,8], in which traumatic osteonecrosis of the femoral head is the avascular necrosis of osteocyte caused by interruption of blood flow in the blood vessels in femoral head, which was due to trauma [9, 10]
The ANFN diagnosis was established by referring to standard of adult femoral head necrosis diagnosis expert consensus (2012 edition) and the diagnosis and treatment of avascular necrosis of the expert advice of diagnostic criteria

Summary

Introduction

Avascular necrosis of the femoral head (ANFH) is a clinical common orthopaedic disease [1,2,3] with very high morbidity. Secondary osteonecrosis of the femoral head could divide into traumatic and nontraumatic osteonecrosis of femoral head [6,7,8], in which traumatic osteonecrosis of the femoral head is the avascular necrosis of osteocyte caused by interruption of blood flow in the blood vessels in femoral head, which was due to trauma [9, 10]. The pathogenesis of steroid-induced ANFN is still unknown. For developing new methods to prevent and treat ANFN, study on the pathogenesis of steroid-induced ANFN is urgent [12, 14, 15]

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Conclusion