Abstract
Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been identified, however the functions of most of these ageing-related proteins are not known. Here, we report the identification of proteins that extended yeast chronological life span (CLS) from a screen of ageing-related proteins. Three of the CLS-extending proteins, Ptc4, Zwf1, and Sme1, contributed to an overall higher survival percentage and shorter doubling time of yeast growth compared to the control. The CLS-extending proteins contributed to thermal and oxidative stress responses differently, suggesting different mechanisms of actions. The overexpression of Ptc4 or Zwf1 also promoted rapid cell proliferation during yeast growth, suggesting their involvement in cell division or growth pathways.
Highlights
Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others
To determine the effects of the overexpressed proteins on yeast chronological life span (CLS), we overexpressed ageing-related proteins in yeast and screened them using outgrowth kinetics assay in a high-throughput manner (Fig. 1a)[1]
For the gene deletion mutants, an aliquot of culture was sampled for outgrowth in a 96-well microplate containing fresh Yeast Peptone Dextrose (YPD) media
Summary
Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others. We report the identification of proteins that extended yeast chronological life span (CLS) from a screen of ageing-related proteins. A CLS screen of deletion mutants revealed genes that significantly extended CLS (ACB1, CKA2, TRM9) and the deletion of these genes promoted heat-shock tolerance[7] Another screen of deletion mutants found that while autophagy defective mutants (ATGmutants) decreased CLS, highly conserved de novo purine biosynthesis pathway defective mutants (the ADE mutants) extended CLS to the same degree as calorie r estriction[8]. In another CLS screen, CYR1 and SCH9 mutants were found to extend CLS and increase thermal and oxidative stress tolerances[9].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
