Screening of rhizobacteria for multi-trait plant growth-promoting ability and antagonism against B. fabae, the causative agent of chocolate spot disease of faba bean

Abstract

This study aimed to isolate and characterize plant growth-promoting rhizobacteria from the faba bean rhizosphere for future inoculum production. For this purpose,127 dissimilar rhizobacterial colonies were isolated. All isolated colonies were tested for plant growth-promoting traits. Based on their multiple plant growth-promoting traits, eight potential isolates were selected and identified GY01, GY03, and GY08 are affiliated with Acinetobacter sp. GY04 and GY05 are affiliated with Chryseobacterium sp. GY06 and GY07 are affiliated with Pseudomonas costantinii and Pseudomonas chlororaphis, respectively; and GY02 is affiliated with the Bacterium strain. All eight isolates were evaluated for their effects on seed germination and vigor index and potential antagonism against Botrytis fabae. Selected isolates showed positive effects on seed germination and vigor index with different potentials. Isolate GY04 resulted in the highest vigor index (501), while isolate GY08 achieved the lowest (218). B. fabae radial growth inhibition was found in all eight isolates. The isolates inhibited the radial growth of the test pathogen with an inhibition efficacy of 72.38 % in GY04 to 25.57 % in GY-03. Generally, the results of this study indicated the potential of these isolates as a microbial inoculant with multiple functions for faba beans.