Screening of rare actinomycetes isolated from natural wetland ecosystem (Fetzara Lake, northeastern Algeria) for hydrolytic enzymes and antimicrobial activities

Abstract

Actinomycetes from unexplored habitats are considered as a promising source for novel bioactive compounds with a broad range of biological activities. A study was carried out to isolate and identify rare actinomycetes producing antimicrobial from a natural wetland. Water samples from Fetzara Lake (North eastern-Algeria) were collected and subjected to rare actinomycetes isolation using different rich media. Eight selected actinomycetes were screened in vitro for hydrolytic enzymes, antibacterial and anticandidal activities. Based on the 16S rRNA sequencing, the eight actinomycetes isolates were categorized into four different rare genera Actinomadura, Nocardia, Nonomuraea and Micromonospora. Interestingly, significant anticandidal and antibacterial activities against both Gram-positive and Gram-negative bacteria were observed. Furthermore, the actinomycetes isolates were able to produce different hydrolytic enzymes with potential industrial and food processing applications such as amylase, cellulase, protease, and lipase. Overall, the study revealed that the selected aquatic rare actinomycetesrecovered from Fetzara Lake presented good candidates to be explored as new sources of bioactive compounds.