Screening of protein crystallization trials by second order nonlinear optical imaging of chiral crystals (SONICC)

Levi M Haupert,Garth J Simpson

doi:10.1016/j.ymeth.2011.11.003

Screening of protein crystallization trials by second order nonlinear optical imaging of chiral crystals (SONICC)

Levi M Haupert, Garth J Simpson

Open Access

https://doi.org/10.1016/j.ymeth.2011.11.003

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Journal: Methods	Publication Date: Nov 17, 2011
Citations: 53

Affiliation: Purdue University West Lafayette

#Second Order Nonlinear Optical Imaging Of Chiral Crystals #Optical Imaging Of Chiral Crystals + Show 8 more

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Abstract

Second order nonlinear optical imaging of chiral crystals (SONICC) is a promising new method for the sensitive and selective detection of protein crystals. Relevant general principles of second harmonic generation, which underpins SONICC, are reviewed. Instrumentation and methods for SONICC measurements are described and critically assessed in terms of performance trade-offs. Potential origins of false-positives and false-negatives are also discussed.

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