Screening of Potential Biosurfactant Producing Bacteria from Tanjung Perak Port, Surabaya

Marlinda Elvina Susanti,Asep Awaludin Prihanto,Maftuch Maftuch

doi:10.21776/ub.jels.2021.011.03.05

Marlinda Elvina Susanti, Asep Awaludin Prihanto + Show 1 more

Open Access

https://doi.org/10.21776/ub.jels.2021.011.03.05

Copy DOI

Abstract

Bacteria are bioremediation agents that are advised to overcome water pollution. This study aims to isolate biosurfactant-producing bacteria for bioremediation from Tanjung Perak Port, Surabaya. The isolates grown in specific media were tested for drop collapse, oil spreading test, and emulsification index. A total of 12 bacterial isolates were isolated from Tanjung Perak seawater contaminated with diesel oil. In the drop collapse test, four isolates had the highest positive score from this test, isolate LU-5, LU-7, LU-9, and LU-11. While in the second test, the oil spreading test, the highest positive score results were obtained in three isolates, isolates LU-2, LU-7, and LU-11. The emulsification index (E24) value showed that isolates LU-7, LU-9, and LU-11 were significantly higher than the others, which was above 10%. This study concludes that in Tanjung Perak, seawater-contaminated oil isolated several bacteria that produce biosurfactants, which have the potential to be developed as bioremediation agents.Keywords: bacteria, bioremediation, biosurfactant, diesel oil

Highlights

Water pollution is a crucial environmental issue that continues to occur, especially in marine waters
The primary source of marine contamination is derived from oil spills both from offshore drilling processes and from activities by ships that cross the ocean [1]
The results of the drop collapse test in this study showed that none of the isolates produced round and stable droplets as in the control

Summary

Introduction

Water pollution is a crucial environmental issue that continues to occur, especially in marine waters. The primary source of marine contamination is derived from oil spills both from offshore drilling processes and from activities by ships that cross the ocean [1]. Tanjung Perak is the second most significant and active port in Indonesia after Tanjung Priok. To this day, Tanjung Perak is a port used as a trading center in eastern Indonesia. Bunkering activities in the port area or refueling diesel oil when ships are more frequently contributing waste oil spills. This activity can harm life resources, disrupt marine activities, including fishing, marine culture, and decrease seawater quality, up to human health problems [2]

Objectives

Methods

Findings

Conclusion