Abstract
An alternative method for the analysis of poly-β-hydroxybutyrate (PHB) in microbial biomass based on FTIR spectroscopy was developed in which a semi-transparent, thin pellet of biomass was made with KBr and scanned by IR spectroscopy, from 400 cm−1to 2000 cm−1. The IR spectra of the target biomass showed a distinct absorbance band of the carboxylic ester group at 1724.03 cm−1. The technique can be used to screen large numbers of microorganisms for PHB production.
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