Screening of Peptides that Control Interaction Between Nucleobases from Peptide Libraries Based on Loop Structures

Abstract

The combinatorial peptide library has been widely used as a useful tool for screening of the bioactive ligands and/or recognition motif against specific molecules [1]. The random peptide libraries, however, generally have considerable conformational flexibility, thus many kinds of conformationally restrained peptide libraries have been reported recently [2]. In this study, we constructed a unique confomationally-restricted peptide library using a loop structure as a structural scaffold and peptide nucleic acids (PNAs) as a recognition site. By using this structure, we carried out the effective screening of the amino acid sequences that control the interaction between nucleobases.