Screening of Peptides bound to Brash Border Membrane Vesicle of Nilaparvata lugens (Hemiptera: Delphacidae)

Abstract

This research aimed to screen peptides which may bind to the brash border membrane vesicle(BBMV) extracted from the gut of rice brown planthopper(Nilaparvata lugens,Hemiptera: Delphacidae).BBMV of N.lugen was extracted with the same method for extracting BBMV from the gut of lepidopteran and dipteran insects.Then two peptides P1Z and P2Z that may bind to N.lugens gut BBMV were screened by the use of phage display library.Structure and function of screened peptides were analyzed by bio-analysis.Nucleotide of P1Z,P2Z and an unbound peptide UBP were fused to the 5' terminus of egfp gene and P1Z/P2Z/UBP-EGFP fusion proteins were prepared.Binding activity of P1Z and P2Z to N.lugens gut BBMV were confirmed by Pull down and Western blot methods.The results showed that by the use of phage display library it is possible to find peptides which can bind to gut BBMV of N.lugens.Structure and function analysis of screened peptides show that the distribution differences of Alpha amphipathic regions and Beta amphipathic regions are the possible factors to affect BBMV binding activity of peptides.The results of this research could help to develop a new approach to block transmission of rice viruses by N.lugen.