Screening of Pectinase Producing Gram Positive Bacteria: Isolation and Characterization

Fouzia Tabssum,Syed Shahid Ali

doi:10.17582/pujz/2018.33.1.11.15

Abstract

Screening of Pectinase Producing Gram Positive Bacteria: Isolation and Characterization

Highlights

Enzymes are biological catalysts, which facilitate the chemical reactions under diverse sets of physicochemical conditions
To enrich the diversity of isolated bacteria that produce pectinase, samples were collected from the soil, water, fruits and vegetables wastes
Zones of hydrolysis produced by the bacterial strains were ranged from 0.9-2.6 mm2 in diameter

Summary

Introduction

Enzymes are biological catalysts, which facilitate the chemical reactions under diverse sets of physicochemical conditions. Enzymes were discovered in middle of nineteen century, and they were first introduced in industrial application by Dr Jokichi Takamine (1894-1914). Many extracellular microbial enzymes are environmental friendly and have industrial applications. There is increasing demand to replace chemical based industries with biotechnological processes involving microorganisms and use of enzymes such as amylases, lipases, xylanases, cellulases, chitinases and pectinases etc. Pectinases are the group of enzymes, which degrade pectin substances. Pectins are the variety of substances, which are present in higher plants and are macromolecules of high molecular mass They are basically the component of plant cell wall and middle lamella, and very thin extracellular layer, which joins the young, cells together. Homogalacturonans are the linear polymers of pectins, which are formed by D-galacturonic acid monomers, which may be methyl esterified and/or acylated. The reported isolates demand additional research inputs to streamline the economical provision of pectinases to food processing units

Materials and Methods

Results and Discussion

Conclusion