Screening of Organochlorine Pesticide and Polychlorinated Biphenyl Residues in Nonfatty Seafood Products by Tandem Solid-Phase Extraction Cleanup

Abstract

Abstract A rapid multiresidue solid-phase extraction (SPE) technique for the isolation and subsequent gas chromatographic (GC) determination of organo-chlorine pesticide and polychlorinated biphenyl (PCB) residues in nonfatty fish, crabmeat, shrimp, and scallops is described. Samples are extracted with acetonitrile, and the extract is subjected to a cleanup using both C18 and Florisil SPE columns. The residues are determined by GC with electron capture detection. Because the injected extracts are free from matrix interferences, the amount of residue present is easily calculated. The average recoveries of 9 spiked (0.01–0.40 ppm) organo-chlorine pesticide residues from 6 different seafood products ranged from 93.8 to 98.4%. The average recoveries of spiked (0.50 ppm) PCB mixtures from 4 different seafood products ranged from 88.5 to 107.2%. This SPE method and the AOAC multiresidue method for organochlorine residues in fish produced comparable results for nonfatty seafood samples containing incurred organochlorine pesticide and PCB residues. The SPE method reduces organic solvent consumption by 95% and hazardous waste by 85% compared with the AOAC method. We were able to carry 10 seafood samples through the extraction and cleanup in less than 2 h by using the SPE method.