Screening of Omega-3 PUFA inhibiting differential gene expression in colorectal cancer cell lines

Abstract

Objective Epidemiological investigation showed that Omega-3 polyunsaturated fatty acid (Omega-3 PUFA) was negatively correlated with the incidence of colorectal cancer, but the specific mechanism was still unclear. The differential genes of Omega-3 PUFA inhibiting metastasis of colorectal cancer were studied and verified by real-time PCR. Methods MTT were used to detect the effect of Omega-3 PUFA on the activity of colorectal cancer cell lines. The optimal concentration and time were determined. The Human HT-12 v4 Expression Bead Chip developed by Illumina was used to detect the differential genes. Real-time PCR was used to verify the results of candidate differential genes. Results The most optimal condition that Omega-3 PUFA inhibited the vitality of HCT116 cell was 30 µg/ml and 48 h, and the inhibition rate was 53.78%. The most optimal condition that Omega-3 PUFA inhibited the vitality of SW480 cell was 30 µg/ml and 48 h, and the inhibition rate was 71.01%. From the experimental group and the control group, 61 common differentially expressed genes were found in the two colorectal cancer cells. Combined with literature, the gene related to metastasis was COL11A2. Real-time PCR verified the results of differential expressed genes consistent with the microarray results. Conclusion Omega-3PUFA significantly inhibites metastasis of colorectal cancer, which relates to COL11A2 gene. Key words: Real time PCR; Omega-3PUFA; Colorectal cancer; Metastasis; Differential genes