Screening of mungbean for drought tolerance and transcriptome profiling between drought-tolerant and susceptible genotype in response to drought stress

Abstract

Mungbean, is a widely cultivated pulse crop in India, experiences severe drought stress during the cultivation period. The mechanism of drought tolerance in mungbean is not well understood. In this presents study we screened 7 widely cultivated mungbean genotypes towards their drought sensitivity at seedling stage and transcriptome sequencing of drought-tolerant and susceptible genotype to understand the drought tolerance mechanism. Our physiological data such as increase in root length, shoot length, fresh weight, dry weight, relative water content (RWC), proline content, MDA content and molecular data in terms of quantitative expression of drought stress responsive genes under 3-d drought stress in mungbean suggests that, K851 seems to be most drought tolerant and PDM-139 as drought susceptible genotype. The transcriptomic study between K-851 and PDM-139 revealed 22,882 differentially expressed genes (DEGs) which were identified under drought stress, and they were mainly mapped to phytohormone signal transduction, carbon metabolism and flavonoid biosynthesis. Out of these, 10,235 genes were up-regulated and 12,647 genes were down-regulated. Furthermore, we found that, the DEGs related to, phytohormone signal transduction, carbon metabolism and flavonoid biosynthesis and they were more induced in K-851. Our data suggested that, the drought tolerant genotype K-851, scavenges the damage of drought stress by producing more amount of osmolytes, ROS scavengers and sugar biosynthesis.