Screening of Marketed Pork for Ceftiofur residues by HPLC And UV-Vis Spectrophotometer

Abstract

In the present study pork samples from markets of Assam, India were collected and analyzed to detect the presence of Ceftiofur residues using a High Performance liquid Chromatography (HPLC) System and a UV-Vis Spectrophotometer. 300 nos. of representative pork samples were collected from different pork markets of Assam. The samples after collection were preserved at -20°C. Analyses of the samples using High Performance Liquid Chromatography with UV-Vis Detector were done as per the method of Oka et al, 1985 while analyses of the same samples using UV-Vis Spectrophotometer were done as per the method of Annapurna et al. ,2009. Ceftiofur residues were extracted with Mc-IIvaine buffer. Solid phase extraction clean up was done with Sep-pak C18 cartridge. Recovery ranged from 83-95% (HPLC) and 5780%(Spectrophotometer).Out of the tested samples, 10 nos of the screened samples were detected to be positive for trace residues of Ceftiofur using Spectrophotometer while 14 nos .of same samples were detected for ceftiofur residue using HPLC method which were well below the MRL value.