Abstract

Currently microalgae are considered as an efficient biological producer of oil.Present study was conducted to explore the lipid yield percentage of three microalgal strains Chlorellasp., Oedogonium sp. and Spirogyra sp. Bligh and Dyer, Soxhlet, Folch and Microwave assistedextraction methods were employed for oil extraction. It was observed that Bligh and Dyer method withChloroform-methanol (1:2, v/v) gave maximum extraction yield (15.46 ± 0.240 %) for Chlorella sp.whereas Folch method with chloroform-methanol (2:1, v/v) gave more lipid yield as compared toBligh and Dyer method i.e 5.53 ± 0.240 % for Spirogyra sp., 13.67 ± 0.240 % and 19.69 ± 0.667 % forOedogonium sp. and Chlorella sp. respectively. Soxhlet extraction resulted in less (14.5± 0.45)percentage yield as compared to Bligh and Dyer and Folch method (16.7± 0.24). Microwave assistedextraction with chloroform- methanol resulted in highest lipid yield (30%) with Chlorella sp.indicating that it contained the maximum lipid content among all the three species.

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