Abstract
In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase). The results showed that the ratio of transparent circle to mycelium circle of Hericium erinaceus was 8.16 (P < 0.01) higher than other strains. The filter paper culture screening test showed that Hericium erinaceus and Macrolepiota procera grew well and showed extreme decomposition of the filter paper. When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm. CMCase activity determination indicated that Coprinus comatus and Hericium erinaceus had the ability to produce CMCase with 33.92 U/L on the 9th day and 22.58 U/L on the 10th day, respectively, while Coprinus comatus and Pleurotus nebrodensis had the ability to produce laccase with 496.67 U/L and 489.17 U/L on the 16th day and 18th day. Based on the results, Coprinus comatus might be the most promising lignocellulose-degrading strain to produce both CMCase and laccase at high levels.
Highlights
Lignocellulose is an abundant and renewable source of carbohydrates that can be converted into value-added products in the earth
Shiraia bambusicola and Pleurotus nebrodensis showed no transparent circle, and Auricularia auricula with 1.41 cm transparent circle became the third inferior candidate, while Pholiota nameko followed by Hericium erinaceus had the largest transparent circles (5.18 cm and 4.95 cm, resp.)
Congo red can bind to polysaccharides like cellulose but cannot bind to saccharides to form red compounds
Summary
Lignocellulose is an abundant and renewable source of carbohydrates that can be converted into value-added products in the earth. It is estimated that 6.0 × 109 tons of lignocellulose are generated by photosynthesis each year all over the world, but only about 20% are used for conversion into energy and food [1]. Chemical and physical methods of pretreating lignocellulosic compounds have been used to expose the underlying cellulose and hemicelluloses such as radicalization, steam explosion, puffing, acid, and alkali [5]. These methods consume high amounts of energy and cause pollutants [6]. It is pressing to find a high-efficiency, energy-saving, and environment-friendly way to break down cellulose and hemicelluloses
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
