Abstract
Thirty (30) isolates of lactic acid bacteria (LAB) from Philippine traditionally‐prepared fermented products, namely: fermented soy‐bean paste, fermented mustard and fermented rice‐fish mixture were studied for their in vitro antimicrobial and cytotoxic activities. Seventeen (17) isolates were identified as Lactobacillus plantarum, while 13 isolates were identified as Enterococcus spp. using 16S rDNA sequences. The phylogenetic tree revealed that there were three clusters of LAB with 100% similarity level. Disc diffusion method was used to determine the antibacterial activity of LAB against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, while the modified agar overlay method was used to determine the antifungal activity of LAB isolates on the yeast Candida albicans, and the dermatophytes Microsporum gypseum, Trichophyton rubrum and Epidermophyton floccosum. The filter‐sterilized LAB culture supernatants were evaluated for their cytotoxicity to mammalian colon cancer cell lines HT‐29 and HCT116, and normal human dermal fibrolasts HDFn using resazurin assay (PrestoBlue™). Colchicine was the positive control. No antimicrobial activity was observed against the bacterial test organisms and the yeast Candida albicans. On the other hand, all of the tested LAB strains were fungicidal for all the test dermatophytes. Cytotoxicity index profiles of all the LAB supernatants tested showed that these were cytotoxic to HT‐ 29 and HCT116 colon cancer cell lines, but were not cytotoxic for the normal HDFn cells. Results provide strong support for the role of the lactic acid bacteria studied for antifungal treatment and anticancer therapy.Support or Funding Information1. DOST‐SEI, Accelerated Science Technology Human Resource Development Program2. Center for Environmental and Natural Science Research, De La Salle University, Taft Avenue, Manila3. De La Salle University, College of Science, Biology DepartmentThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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