Screening of isolated and collection strains of cyanobacteria on productivity for determining their biotechnological potential

Abstract

Cyanobacteria produce a wide range of metabolites, such as proteins, carbohydrates, carotenoids,vitamins and lipids which can be used as potential food sources for human and animals, in pharmaceutical and cosmetic industries and also as energy source. It is known that the technology of using cyanobacteria as fuel raw materials makes one of the central places among the approaches of modern alternativeenergy. To date, the possibility of using cyanobacteria in wastewater treatment with the further use ofbiomass for obtaining biodiesel fuel is being considered. For their application in biotechnology the strainscreening for producers is needed. Prospectivity of types and species for use in biotechnology is firstlydetermined by their productivity. The article presents data of screening of collection and isolated strainsof cyanobacteria: Cyanobacterium sp. B-1200, Cyanobacterium aponium IPPAS B-1201, Synechococcuselongatus 7942, Anabaena variabilis R-I-5 and Nostoc calsicola RI-3. Comparative analysis of constantfluorescence indices, cell growth rate and accumulation of dry weight in cyanobacteria was carriedout. Thus, as a result of investigations, the greatest optical density was noted in Anabaena variabilisR-I-5 strains (1,191) and Cyanobacterium sp. B-1200 (1,281). Fluorescence indices also confirmed thehigh photosynthetic activity of cyanobacteria Cyanobacterium sp. B-1200 (26101 rel.un.) and Anabaenavariabilis R-I-5 (25054 rel.un). Also, investigated strains Anabaena variabilis R-I-5 and Cyanobacteriumaponium IPPAS B-1201 were characterized by relatively high biomass accumulation – 1,19 g/l и 1,36g/l correspondingly. According to obtained data, it was established that cyanobacteria Cyanobacteriumsp. B-1200 and Anabaena variabilis R-I-5 have a relatively high values of growth rate, fluorescence andbiomass yield which determine their high productivity. Thus, in the result of screening strains Cyanobacterium stanieri B-1 and Anabaena variabilis R-I-5 were selected for further investigations of theirphysiological and biochemical properties with the purpose to determine potential producers of valuablemetabolites for biotechnology.Key words: screening, cyanobacteria, biomass, fluorescence, biotechnological potential