Screening of Indonesian rice (Oryza sativa L.) genotypes against salinity stress using NaCl under hydroponic condition

Abstract

The development of rice in saline soils was constrained by the limited number of genotypes suitable to be developed in the area and also the lack of germplasm as a gene donor for saline soil tolerant properties. The study aims to select rice genotypes that are tolerant of salinity stress at the level of germination and hydroponic production. The research on the level of germination carried out at the University of Hasanuddin Tissue Culture Laboratory and the level of production carried out at the Screen House in Tamalanrea District, Makassar. Trials were set using a split plot design with NaCl concentration set as the main plot and rice genotypes as the subplots. Four NaCl were used in the germination trial 0, 3, 6, and 9 g L−1, while NaCl concentration used in the production trial were 0 g L−1 and 6 g L−1. A total of 25 rice genotypes was tested. The results show that genotypes that were tolerant to salinity stress at the germination test with the concentration of 6 g L−1 NaCl were Inpari 43, Limboto, Inpago 10, IR 58443-6B-10-3, Inpari 35, Inpari 4, MSP 8 and Cigeulis. Whereas the rice genotype with the potential to be tolerant to salinity stress, indicated with production of > 15 g per plant at the 6 g L−1 NaCl concentration, were Inpari 35 (21.53 g per plant), Inpari 43 (20.24 g per plant), Pokali (19.39 g per plant), and IR 58443-6B-10-3 (18.87 g per plant).