Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization.

Jiajun Li,Kangcheng Pan,Abdul Khalique,Dongmei Zhang,Junrui Wang,Xueqin Ni,Qihui Luo,Dong Zeng,Miao Yang,Wanqiang Li,Zhenhua Wang,Bo Jing,Yan Zeng

doi:10.1371/journal.pone.0235476

Jiajun Li, Kangcheng Pan + Show 11 more

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https://doi.org/10.1371/journal.pone.0235476

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Abstract

To explore the molecular mechanism of the effect of Bacillus cereus PAS38 on the immunity of broilers, sixty 7-day-old broilers were divided into two groups with three replicates. The control group was fed with basal diet, and the treatment group was fed with basal diet containing Bacillus cereus PAS38 1×106 CFU/g. Thymus and bursa of fabricius were taken from two groups of broilers at the age of 42 days, total RNA was extracted, differential gene library was constructed by SSH technology, and immune-related differential genes were screened. Then, we used siRNA to interfere with the expression of some differential genes in the original generation lymphocytes of broiler blood to detect the change of cytokines mRNA expression level. A total of 42 immune-related differentially expressed genes were screened, including 22 up-regulated genes and 20 down-regulated genes. When 7 differentially up-regulated genes associated with enhanced immune function were interfered with in lymphocytes, some immune-promoting cytokines were down-regulated. These results showed that Bacillus cereus PAS38 might up-regulate the expression of JCHAIN, PRDX1, CD3E, CDK6 and other genes in immune organs of broilers, thereby affecting the development of immune organs, the expression of various cytokines and the transduction of immune signals, improving the immune capacity of broilers.

Highlights

The most prominent feature of probiotic Bacillus is that it can produce stress-resistant spores under adverse environmental conditions such as high temperature, ultraviolet radiation and chemical reagents
Double-stranded cDNA was synthesized by different PCR cycles, and 1.2% agarose gel electrophoresis was shown in Fig 1 (S1 Fig)
This study found that, after the interference of IGF1R, the expression of TLR4, IL-6, MYD88 and IL-1B were significantly down-regulated, which suggests that IGF1R may regulate the expression of certain cytokines by affecting the development of immune organs and the number of lymphocytes in broilers, play a positive regulatory role in the development of the immune system

Summary

Introduction

The most prominent feature of probiotic Bacillus is that it can produce stress-resistant spores under adverse environmental conditions such as high temperature, ultraviolet radiation and chemical reagents. Screening of different genes of broilers fed bacillus cereus based on suppression subtractive hybridization. Techonology Innovation Research Team in the University of Sichuan Province (Award Number: KM406183.1), Pan KC received the funding. (http:// kjt.sc.gov.cn/) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript Techonology Innovation Research Team in the University of Sichuan Province (Award Number: KM406183.1), Pan KC received the funding. (http:// kjt.sc.gov.cn/) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

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