Abstract
BackgroundBased on the molecular expression level, this paper compares lncRNA and mRNA expressions respectively in peripheral blood samples of the patients after SCI with NP and without NP, and screens disease-related biomarkers related to NP after SCI in peripheral blood samples of patients.MethodThe expression spectrum of 25 human peripheral blood samples (12 samples of refractory NP patients after SCI) was downloaded and data were normalized. Screening of GO annotations significantly associated with significant differentially expressed mRNAs and significant involvement of the KEGG pathway. The WGCNA algorithm was used to screen for modules and RNAs that were significantly associated with disease characterization. A co-expression network was constructed to extract the genes involved in the disease pathway from the co-expression network, construct a network of SCI pain-related pathways, and screen important disease-related biomarkers. Quantitative real-time PCR was used to detect the mRNA expression of hub genes.ResultsData were normalized and re-annotated by detection of platform information, resulting in a total of 289 lncRNA and 18197 mRNAs. Screening resulted in 338 significant differentially expressed RNAs that met the threshold requirements. Differentially expressed RNAs were significantly enriched with the brown and magenta modules. Six KEGG signaling pathways were screened in the co-expression network, and three KEGG pathways with direct neuropathic pain were identified. The expression levels of E2F1, MAX, MITF, CTNNA1, and ADORA2B in the disease group were all significantly upregulated (p < 0.01). Compared with the normal group, the expression of OXTR was upregulated.ConclusionWe speculate that there are 7 genes and 2 lncRNAs directly involved in the pain pathway: E2F1, MAX, MITF, CTNNA1, ADORA2B, GRIK3, OXTR, LINC01119, and LINC02447. These molecules may be important for NP after SCI.
Highlights
Spinal cord injury (SCI) refers to the damage of the spinal cord due to various pathogenic factors and is a common clinical disease in orthopedics [1]
Six Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways were screened in the co-expression network, and three KEGG pathways with direct neuropathic pain were identified
We speculate that there are 7 genes and 2 lncRNAs directly involved in the pain pathway: E2F1, MAX, MITF, CTNNA1, ADORA2B, GRIK3, oxytocin receptor gene (OXTR), LINC01119, and LINC02447
Summary
Spinal cord injury (SCI) refers to the damage of the spinal cord due to various pathogenic factors and is a common clinical disease in orthopedics [1]. The formation of nerve scars hinders the growth of nerve fibers, and exogenous factors hinder regeneration after spinal cord injury [4]. In recent years, a large number of studies have shown that endogenous factors may have a greater effect on nerve repair after spinal cord injury [5]. Patients with NP are unbearable, but long-term pain affects their sleep, work, and life [6]. Research on this field is currently based on animal models, but research reports on human whole blood are rare. Based on the molecular expression level, this paper compares lncRNA and mRNA expressions respectively in peripheral blood samples of the patients after SCI with NP and without NP, and screens diseaserelated biomarkers related to NP after SCI in peripheral blood samples of patients
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