Screening of differential microRNA and prediction of target genes between human immunodeficiency virus-Burkitt lymphoma and diffuse large B cell lymphoma

Abstract

Objective To screen the differential microRNA (miRNA) in human immunodeficiency virus (HIV) related Burkitt lymphoma (BL) and diffuse large B cell lymphoma (DLBCL). Methods Five freshly frozen tissue samples of BL and 3 freshly frozen tissu samples of DLBCL, 19 paraffin specimens of BL and 15 paraffin specimens of DLBCL were collected from Shanghai Public Health Clinical Center. Agilent human miRNA microarrays were employed to detect the miRNA expressions in fresh frozen BL tissues and fresh frozen DLBCL tissues, and to find out differential miRNA. SmartRNAplex™ miRNA was employed to verify the expressions of crucial miRNA in BL formalin fixed and paraffin-embedded tissues and DLBCL FFPET. Bioinformatics methods were used to predict the target genes of the crucial miRNA. Results Compared with DLBCL group, 42 differential miRNA were detected in BL group. Among them, 28 miRNA were up-regulated and 14 miRNA were down-regulated in BL group. According to positive control in eukaryote and high-expression molecular contributing to the emergence of tumor, 5 crucial miRNA were selected from 28 up-regulated miRNA in BL group for validation. The result was consistent with that of Agilent human miRNA microarrays. Compared with the DLBCL group, 5 crucial miRNA were all up-regulated in BL, which were miRNA-16-2-3p, miRNA-20a-3p, miRNA-130b-3p, miRNA-185-5p and miRNA-423-5p (t=2.7151, 2.539, 2.750, 4.004, and 3.625, respectively, all P<0.05). The corresponding target genes of miRNA-16-2-3p might be CTNND2 and RAD21.The target genes of miRNA-20a-3p might mainly be DYRK1A and GPAM. The target genes of miRNA-130b-3p might mainly be IRF1, DICER1 and PTEN. The target genes of miRNA-185-5p might mainly be VEGFA, NFATC3 and SEC24C.The target genes of miRNA-423-5p might mainly be PA2G4 and PNKD. Conclusions There are significantly differentially expressed miRNA between BL and DLBCL tissues. These miRNA are expected to provide new molecular markers for diagnosis and differential diagnosis of BL and DLBCL. Potential target genes of crucial miRNA are related with cell survival, proliferation, differentiation, apoptosis and carcinogenesis, etc, which may play important roles in the origination and progress of BL. Key words: Human immunodeficiency virus; Burkitt lymphoma; Lymphoma, large B-cell, diffuse; MicroRNA; Clinical pathology