Abstract
Eleven different Fusarium species were isolated from various infected plant materials and screened to select a potential species for the synthesis of silver nanoparticles. All the isolates were identified on the basis of cultural and microscopic characteristics using Fusarium identification keys. For the confirmation of preliminary identified isolates of Fusarium species, online BLAST analysis was carried out. All the eleven species demonstrated the ability for synthesis of silver nanoparticles. This was confirmed by UV-Vis spectroscopy, which gave characteristic peak around 420 nm. Further confirmation of silver nanoparticles was carried out using nanoparticles tracking analysis (NTA), zeta potential, photon correlation spectroscopy (PCS), powder X-ray diffractometry (XRD) and transmission electron microscopy (TEM). The smallest size of silver nanoparticles was synthesized by F. oxysporum (3-25 nm) and largest size silver nanoparticles were synthesized by F. solani (3-50 nm).
Highlights
Nanotechnology is a multidisciplinary field linked with different disciplines of science and technology likeVol 24, No 12, 2013 materials
Cultural and microscopic characteristics confirmed the identity of 11 different species of Fusarium viz., F. graminearum, F. solani, F. oxysporum, F. culmorum, F. scirpi, F. tricinctum, F. acuminatum, F. semitectum, F. proliferatum, F. equiseti, F. moniliforme
On the basis of UV-Vis spectrophometric analysis and stability of silver nanoparticles, six species, viz., F. graminearum, F. solani, F. oxysporum, F. culmorum, F. scirpi and F. tricinctum were selected for the further characterization (XRD, nanoparticles tracking analysis (NTA), transmission electron microscopy (TEM), and ZetaSizer)
Summary
Nanotechnology is a multidisciplinary field linked with different disciplines of science and technology likeVol 24, No 12, 2013 materials. We screened 11 different Fusarium species for the selection of species having high potential for the synthesis of silver nanoparticles. Transmission Electron Microscopy (TEM) To determine the size and morphological variation of silver nanoparticles synthesized by different Fusaria species, the TEM analysis was carried out using Carl Zeiss
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