Screening of different species of Linum GenRes by lectin profile

Abstract

In recent years, cellular glycans are increasingly recognized as a universal basis for the storage and transmission of biological information. Lectins, carbohydrate-binding proteins, play a key role in this process. A very interesting model for studying the processes of intercellular interaction and recognition is self-incompatibility in plants. This is one of the main mechanisms by which angiosperms prevent inbreeding and promote cross-pollination. Heteromorphic incompatibility is the most interesting type of self-incompatibility, because in heterostyle species S-genes are associated with morphological features of flowers. The genus Linum is very interesting in terms of studying the mechanisms of pollination. Among the representatives of this genus there are both self-pollinating species (homostyled species) and cross-pollinating species (heterostyled species). The aim of the study was to analyze the lectin profile in the pistils and stamens of both flower morphs of three model species, which are characterized by different types of self-incompatibility: L. grandiflorum Desf., L. perenne L. and L. thracicum Degen. It was found that the lectin profile of androceum and gynoecium of heterostyled Linum species consists of 40 fractions regardless of genotype. It was found that lectins of L. perenne have the highest activity, and lectins of L. grandiflorum has the lowest. The activity of lectins of L. thracicum occupies an intermediate position. The relationship between the level of lectin activity and the type of self-incompatibility has been identified, suggesting that lectins may be involved in the regulation of self-incompatibility. A method of complete extraction and purification of lectins of different types of Linum by affinity chromatography using galactose and glucose-containing carriers has been developed.