Screening of different Fe (II) and Fe (III) complexes at the stage of rhizogenesis in vitro of gooseberry plants

Abstract

During clonal micropropagation of the Pink-2 (Rosovy-2) gooseberry cultivar at the stage of rhizogenesis, the efficiency of modification of the media with mineral salts according to Quoirin-Lepoivre (QL) was shown by replacing iron, which is standardly used in the form of FeSO4×7H2O together with Na2EDTA, with chelated forms with carboxyl-containing ligands Fe(III)-EDTA and Fe(III)-DTPA and the organophosphate complexone Fe(II)-HEDP. On the 45th and 60th days of subculturing, the distribution in descending order of the impact of chelate iron compounds on the rooting rate of the studied gooseberry plants was as follows: Fe(III)-EDTA>Fe(III)-DTPA> Fe(II)-HEDP> Fe(III)-EDDHA>Fe(III)-HEDP. On the 60th day of subculturing in the best variants of the experiment, the rooting rate of gooseberry microcuttings of cultivar Pink-2 was 86.7-100% compared to 60% in the control variant.