Screening of Diatom Strains and Characterization of Cyclotella cryptica as A Potential Fucoxanthin Producer.

Bingbing Guo,Bin Liu,Bo Yang,Jin Liu,Xue Lu,Feng Chen,Peipei Sun

doi:10.3390/md14070125

Abstract

Fucoxanthin has been receiving ever-increasing interest due to its broad health beneficial effects. Currently, seaweeds are the predominant source of natural fucoxanthin. However, the disappointingly low fucoxanthin content has impeded their use, driving the exploration of alternative fucoxanthin producers. In the present study, thirteen diatom strains were evaluated with respect to growth and fucoxanthin production potential. Cyclotella cryptica (CCMP 333), which grew well for fucoxanthin production under both photoautotrophic and heterotrophic growth conditions, was selected for further investigation. The supply of nitrate and light individually or in combination were all found to promote growth and fucoxanthin accumulation. When transferring heterotrophic cultures to light, fucoxanthin responded differentially to light intensities and was impaired by higher light intensity with a concomitant increase in diadinoxanthin and diatoxanthin, indicative of the modulation of Diadinoxanthin Cycle to cope with the light stress. Taken together, we, for the first time, performed the screening of diatom strains for fucoxanthin production potential and investigated in detail the effect of nutritional and environmental factors on C. cryptica growth and fucoxanthin accumulation. These results provide valuable implications into future engineering of C. cryptica culture parameters for improved fucoxanthin production and C. cryptica may emerge as a promising microalgal source of fucoxanthin.

Highlights

Fucoxanthin, a specific non-provitamin A carotenoid, is mainly found in brown seaweeds, diatoms and golden algae [1]
We demonstrated that light supply affected the growth and fucoxanthin production of
We found that nitrate supply to modified SK medium benefited C. cryptica cryptica growth to a higher biomass concentration in line with growth leading to a leading higher biomass concentration (Figure 4)

Summary

Introduction

Fucoxanthin, a specific non-provitamin A carotenoid, is mainly found in brown seaweeds, diatoms and golden algae [1]. Fucoxanthin harbors a very unique structure with an allenic bond, a conjugated carbonyl and a 5,6-monoepoxide [2]. This structure endows fucoxanthin with a variety of biological activities, including anti-obesity and anti-diabetes [3,4,5,6], anti-cancer [7,8,9,10,11], anti-allergic [12,13], anti-inflammatory [14,15], anti-oxidation [14], and anti-osteoporotic activities [14]. The marine algae diatoms, which grow rapidly, have high fucoxanthin content, and perform robustly in controlled bioreactors [25,26,27], are considered as potential alternative producers of

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