Abstract
Chrysanthemum stunt viroid (CSVd) belonging to Pospiviroidae was found prevailing in some of the chrysanthemum plant samples collected from various geographical regions of India. In this study 70% cultivars of chrysanthemum were tested positive for CSVd by reverse transcription polymerase chain reaction (RT-PCR) and DNA-RNA hybridisation. The viroid was found to be mechanically transmissible to in vitro grown healthy chrysanthemum cv. Regol Time. The RT-PCR amplified product of CSVd was sequenced (348 bp length) and was directly used as a probe for hybridisation. Isolates from Palampur, Mandi, Karnataka, Bangalore and Sikkim showed positive results for CSVd. This analysis shows that CSVd is prevailing in these regions of India.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
