Abstract
Screening tests are described for the development of chemiluminescence systems (oxidizing systems) capable of detecting biological organic compounds. The light emission depends strongly on the oxidizing systems employed. Acidic permanganate system gives rise to light emission for many compounds, including catechols, catecholamines, triphenols and indoles. The following oxidizing systems led specifically to chemiluminescence for hydroquinone, adrenaline or phenylpyruvic acid: 10 −1 M thiosulphate with 10 −1 M sodium hydroxide and 10 −4 M Ag (I), 0.3 % hydrogen peroxide with 10 −3 M sodium hydroxide/50% acetonitrile and 10 −4 M Fe (II), and 0.3% hydrogen peroxide with 10 −2 M sodium hydroxide/10 −2 M didodecyldimethylammonium bromide and 10 −4 M Co(II), respectively.
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