Abstract
Background: Intestinal capillariasis is a disease caused by Capillaria philippinensis. Human infectionsbecame more prevalent in many countries including Egypt. This nematode is related to Trichinella andTrichuris species, all of them belong to Trichinelloidea superfamily. Diagnosis of intestinal capillariasismay be missed by stool examination.Objective: This study aimed to use enzyme-linked immunosorbent assay (ELISA) and immunoblotting inscreening for intestinal capillariasis as a practical and rapid diagnostic test.Subjects and Methods: Trichuris muris and Trichinella spiralis adult worms were isolated from infectedmice and crude antigens were prepared. The protein content for both adult worm extracts was determined.Human blood samples were collected from 20 capillariasis patients, 20 control individuals and 10 W.bancrofti infected patients. The study evaluated cross-reactivity between T. spiralis and T. muris antigensand sera from cases infected with C. philippinensis using ELISA and immunoblotting.Results: In ELISA, T. muris crude antigen cross reacted with 100% of capillariasis sera with 100%sensitivity and specificity and cross reacted with 10% of sera from bancroftian filariasis. T. spiralis crudeantigen cross reacted with 50% of capillariasis sera, and 9% of sera from bancroftian filariasis. Neither T.muris nor T. spiralis crude antigens reacted with sera from control group. Immunoblotting results showedthat IgG antibodies from control group didn't recognize specific proteins in T. muris antigen, while in W.bancrofti group, only one band in one sample appeared (100-135 kDa). IgG antibodies from capillariasiscases recognized multiple common protein bands (35-180 kDa). IgG antibodies from capillariasis, controland W. bancrofti sera did not recognize specific proteins in T. spiralis crude antigen.Conclusion: Antigens from T. muris and T. spiralis can be used successfully to detect infection with C.philippinensis using serum samples of cases by ELISA. Crude antigen of T. muris gave better results thanthat of T. spiralis. Immunoblotting can be used for diagnosis of capillariasis by using crude antigen of T.muris.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
