Screening of bacterial populations in crop rotations with different proportion of cereals

Abstract

Abstract Terminal restriction fragment length polymorphism was used to determine the bacterial community diversity in crop rotations with different proportion of cereals (40%, 60% and 80%) and various fertilisation treatments (F1 - mineral fertiliser amendment + the application of organic fertiliser Veget® and F2 - mineral fertilisation) sampled on two dates (July and October 2012). No statistically significant differences in the number of terminal restriction fragments were detected by Fisher´s least significant difference between two types of fertilisation or among samples with different proportion of cereals. In contrast, statistically significant differences were detected between samples collected in July and October, when in October there was a 43.5% reduction in the number of bacterial species in comparison with July. Principal component analysis as well as cluster analysis showed that a higher similarity in composition of bacterial communities was present among all soil samples collected in July and two samples collected in October. Other samples collected in October were separated from each other. The results of this study demonstrated that bacteria abundance and community composition were not affected by the proportion of cereals and fertiliser used.