Screening of Bacterial and Fungal Biota Associated with Oreochromis niloticus in Lake Manzala and Its Impact on Human Health

Mahmoud M M Zaky,Mohsen E Ibrahim

doi:10.4236/health.2017.94050

Abstract

In this study fish samples were investigated for Bacteria and Fungi, in fish parts, such as intestine, gills and skin. Bacterial counts of Total viable bacteria (T.V.B) reached more than 9.5 × 105 cfu and faecal coliforms (F.C) reached 1.2 × 105 cfu. Different species were identified, Using API technology. Escherichia coli comes first by being recording thirty number case of isolation constituting 36%, it followed by Proteus mirabilis which is represented by twenty four number case of isolation accounting for 30%. Other taxa viz: Klebsiella pneumonia, Citrobacter freundii, Providencia stuartii and Erwinia sp. came next according to their case number of isolation, they represented only by 7%. They showed different antibiotics with different inhibitory concentrations, resistance pattern to gentamycin 10 μg, Penicillin G, tetracycline 30 μg, Ampicillin 10 μg, Ampicillin 30 μg, chloramphenicol (C) 30 μg, Rifampicin 25 μg, Streptomycin 5 μg, cefotaxime 15 μg. Mycological investigation revealed the presence of variety of fungal species, particularly, toxigenic species, such as, Aspergillus spp, Penicillium spp and Fusarium spp. These results are indication of the spoilage of fishes which caught from Lake Manzala and are risky for human consumption and hazardous to human health in this important area of Egypt, due to the high pollution condition of Lake Manzala.

Highlights

Fish and other free-swimming marine animals do not usually carry those organisms generally considered to be typical of the mammalian microflora, including Escherichia coli, the “faecal coliforms”, and enterococci
Recorded bacteria belong to six genera Table 3; of which Escherichia coli comes first by being recording thirty number case of isolation constituting 36%, it followed by Proteus mirabilis which is represented by twenty four number case of isolation accounting for
Our data revealed that recorded bacteria belong to six genera of which Escherichia coli comes first by being recording thirty number case of isolation constituting 36%, it followed by Proteus mirabilis which is represented by twenty four number case of isolation accounting for 30%

Summary

Introduction

Fish provide a large percent of animal protein consumed by the world population. In tropical and subtropical countries, 60 percent of the people depend on fish for 40 percent or more of their protein [1]. Bacterial and fungal contamination of fish is considered the main cause of signs of spoilage as off flavor and unpalatable taste and it may constitute a public health hazard as well as many of economic losses [2] [3] [4]. Additional contamination may occur during the harvesting, handling or processing of the fish, during the storage and transportation “Fish and Fishery Products Hazards and Controls Guidance, Fourth Edition—April 2011”

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