Abstract
Myxobacteria are a class of prokaryotes with complex multi-cellular behavior and could produce various bioactive materials. Forty-eight strains stored in Key Lab of Microbial Diversity Research and Application of Hebei Province were screened by MTT methods with L1210, Hela and normal diploid human embryonic lung fibroblast cell lines as screening models. The results demonstrated that most of the strains could inhibit cancer cells. The inhibition rates to L1210 and Hela are about 78.5% and 59%, respectively. Strain 910018 and 920036 showed higher inhibition activity to cancer cells L1210 and Hela in compare with lower inhibition activity to normal diploid human embryonic lung fibroblast cell line. Therefore, the extracts from the two strains have the potentials as anticancer drugs and were undertaken in vivo anticancer experiments. The results showed that strain 910018 and 920036 could inhibit mice transplanted liver cancer cells H22. The inhibition rate of low doses group of strain 910018 is 69.37% and that of strain 920036 is 54.98%. Therefore, the two strains could be good candidates for producing of anticancer drugs.
Highlights
IntroductionMyxobacteria are a class of prokaryotes with complex multi-cellular behavior and could produce various bioactive materials
The 48 Myxobacteria strains used in this study were isolated and stored by Key Lab of Microbial Diversity Research and Application of Hebei Province
Anticancer materials were screened by MTT method using 96-well plate, with L1210, Hela and MRC-5 cell lines as screening models
Summary
Myxobacteria are a class of prokaryotes with complex multi-cellular behavior and could produce various bioactive materials. Forty-eight strains stored in Key Lab of Microbial Diversity Research and Application of Hebei Province were screened by MTT methods with L1210, Hela and normal diploid human embryonic lung fibroblast cell lines as screening models. The results demonstrated that most of the strains could inhibit cancer cells. Strain 910018 and 920036 showed higher inhibition activity to cancer cells L1210 and Hela in compare with lower inhibition activity to normal diploid human embryonic lung fibroblast cell line. Many studies reported that the secondary metabolites of Myxobacteria have great potentials for application (Guo, 2007; Guo et al, 2007). Myxobacteria were a group of microbe resources that need to be further studied. In order to research new medicine, more attention should be paid to secondary metabolites of Myxobacteria
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