Screening of antibiotic resistance genes in pathogenic bacteria isolated from tiny freshwater shrimp (Macrobrachium lanchesteri) and "Kung Ten", the uncooked Thai food.

Kanittada Thongkao,Yuttana Sudjaroen

doi:10.5455/javar.2020.g397

Kanittada Thongkao, Yuttana Sudjaroen

Open Access

https://doi.org/10.5455/javar.2020.g397

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Abstract

Objective:This study aimed to isolate and identify of pathogenic bacteria in tiny freshwater shrimp (Macrobrachium lanchesteri) and in Kung Ten, which is an unusual Thai cuisine that eaten alive shrimp directly. Antimicrobial susceptibility test and identification of antibiotic resistance genes for isolated bacteria were conducted.Materials and Methods:Eighty of fresh shrimp samples and forty of Kung Ten salads were collected from four fresh markets, which were located in Bangkok and Nonthaburi province (N = 120). The isolation, identification, and antimicrobial susceptibility test of pathogenic bacteria were done following the Clinical and Laboratory Standards Institute guidelines. Antibiotic-resistant bacteria were screened for β-lactamase relating genes, such as AmpC (MOX and ACC genes), blaCTX-M, and Int1 genes.Results:The number of bacterial isolates in tiny freshwater shrimp and Kung Ten salad was 136 and 65, respectively. Aeromonas caviae, A. hydrophilla, Proteus penneri, Proteus vulgaris, and Klebsiella pneumoniae were commonly found. Ampicillin, amoxicillin/clavulanic, cefuroxime, tetracycline, and trimethoprim/sulfamethoxazole resistance were observed, and common antibiotic-resistant bacteria were A. caviae, P. vulgaris, Enterobacter Aerogenes, and K. pneumoniae. A. caviae, P. penneri, K. Pneumoniae, and A. hydrophilla were positive for MOX gene; blaCTX-M, and Int1 genes; ACC and Int1 genes; and ACC gene, respectively.Conclusion:Raw or uncooked shrimps in Kung Ten salad may a risk in foodborne diseases due to positive for pathogenic bacterial isolates. However, hygienic control on food preparation is difficult to apply because of the difficulty of changing in local Thai food behavior.

Highlights

The consumption of raw or half-cooked meat and fishery products may cause human illness
Raw or uncooked shrimps in Kung Ten salad may a risk in foodborne diseases due to positive for pathogenic bacterial isolates
Screening of antibiotic resistance genes in pathogenic bacteria isolated from tiny freshwater shrimp (Macrobrachium lanchesteri) and “Kung Ten”, the uncooked Thai food

Summary

Introduction

The consumption of raw or half-cooked meat and fishery products may cause human illness. Screening of antibiotic resistance genes in pathogenic bacteria isolated from tiny freshwater shrimp (Macrobrachium lanchesteri) and “Kung Ten”, the uncooked Thai food. Tiny freshwater shrimp (Macrobrachium lanchesteri) is a small aquatic crustacean (15–56 mm), rostrum straight or weakly curving dorsal, barely reaching or extending slightly beyond antennal scale, usually 8 to 9 of dorsal and usually 3 to 4 of ventral teeth [13]. Their productivity is part of the food chain that may be an important economic in human food and aquaculture. Isolation, identification, and antimicrobial susceptibility of pathogenic bacteria were conducted and were further screened for β-lactamase relating genes, such as AmpC (MOX and ACC genes), blaCTX-M, and Int genes

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Results and Discussion

Conclusion

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