Screening of antibiotic-producing Streptomyces from marine sediments of Bangladesh

Shajeda Akter Nishat,Mohammad Morshad Alam

doi:10.3329/bsmmuj.v10i4.34403

Abstract

<p>Antimicrobial resistance is a rising concern in the treatment of infectious diseases and the discovery of potential antimicrobial compounds is needed to combat against it. The focus of this study was the in vitro antimicrobial activities of Streptomyces obtained from the soil samples collected from different places of Cox’s Bazar, Bangladesh. A total of 156 isolates was obtained from thirty soil samples using two selective media namely yeast malt agar and starch casein agar. The isolates were morphologically distinct on the basis of spore mass color, reverse slide color, aerial and substrate mycelia formation and production of diffusible pigment. Among the isolates, 12 exhibited good antimicrobial activity against the tested micro-organisms. Isolates were subjected to biochemical characterization and identified as Streptomyces spp. The results suggest that the Streptomyces species could be a promising source for potential antibacterial agents.</p>

Highlights

1 The demand for new antibiotics continues to grow due to the rapidly emerging of multiple antibiotic resistant pathogens causing life-threatening infection and nature still remains the richest and the most versatile source of new antibiotics.[2,3,4]
A total of 156 isolates were recovered from the marine soil samples collected from the different location of the Cox’s Bazar in Bangladesh
The collected samples were varied in their physicochemical, biochemical and biological nature, which consisted of sediment, seawater and intestinal tract of shrimp. Pretreatment methods such as heat treatment, air drying and serial dilution were done which was an important step for reducing contamination and enhancing the isolation of Streptomyces spp

Summary

Introduction

The discovery and application of antibiotics in the treatment of bacterial diseases had been a noteworthy medical success of the 20th century. 1 The demand for new antibiotics continues to grow due to the rapidly emerging of multiple antibiotic resistant pathogens causing life-threatening infection and nature still remains the richest and the most versatile source of new antibiotics.[2,3,4] Natural products having novel structures have been observed to perform inherent biological activities. 1 The demand for new antibiotics continues to grow due to the rapidly emerging of multiple antibiotic resistant pathogens causing life-threatening infection and nature still remains the richest and the most versatile source of new antibiotics.[2,3,4] Natural products having novel structures have been observed to perform inherent biological activities. Soil Actinomycetes are of special interest because of their known property to produce chemically diverse compounds with a wide range of biological activities.[5] A huge number of currently used antibiotics including erythromycin, streptomycin, rifamycin, and gentamycin, are all products isolated from soil Actinomycetes.[6] The two major groups of soil Actinomycetes that serve as important sources of antibiotics are Streptomyces and Micromonospora. It has been stated that Streptomyces account for about 80% of the total antibiotic products; while Micromonospora closely follows with less than one-tenth as much as Streptomyces.[7]

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Conclusion