Screening of amylolytic and cellulolytic yeast from Dendrobium spathilingue in Bali Botanical Garden, Indonesia

Abstract

The objective of this research is to get the isolates of yeast which have the potential to produce amylase and cellulose enzymes. Cellulase and amylase enzymes are the examples of the types of enzymes that play an important role in biotechnology and industry. Dendrobium spathilingue flower samples were obtained from Bali National Park, Indonesia. Isolation is done by using direct & washing method on Potato Dextrose Agar (PDA) medium. A total of 180 isolates of yeast were obtained from isolation. Based on the varied color and texture characters of the colony, as many as 30 selected representative yeast isolates were selected for Selection Yeast Cellulose & Amylase. Cellulolytic yeast screening process was performed using Carboxyl Methyl Cellulose (CMC) medium, obtained by 2 isolates of cellulolytic potential yeast, the W.3.8 yeast isolate had high cellulolytic potential, while the amylolytic yeast screening process was performed using a medium of Yeast Peptone Starch (Soluble YPSS), obtained by 17 potential amylolytic isolates, the yeast isolate W.1.5 has a high amylolytic potential. Cellulolytic and amylolytic activity is known by the occurrence of clear zones surrounding the colony. The yeast isolates W.3.8 and W.1.5 have white, mucoid texture, glossy surface, mountain elevation, and flat edges.