Screening of a pyrene-degrading bacterium and its degradation effect on polycyclic aromatic hydrocarbons oxidation

Abstract

Enrichment culture method, A highly efficient functional bacterium B4 was isolated from polycyclic aromatic hydrocarbon contaminated soil by gradient domestication with pyrene as a single carbon source. The bacterium has strong tolerance to high concentration of polycyclic aromatic hydrocarbons and good degradation ability in a certain concentration range. The degradation rate of pyrene by the strain was 85% under the condition of pyrene concentration of 100 mg/L and inoculation amount of 5%. The degradation effects of the strain on polycyclic aromatic hydrocarbons (PAHs) such as fluorene, naphthalene, fluoranthene and phenanthrene were also investigated. The results showed that the strain B4 had good broad-spectrum degradation performance for PAHs.