Screening of α-glucosidase inhibitors from green tea extracts using immobilized enzymes affinity capture combined with UHPLC-QTOF MS analysis

Abstract

An applicable affinity capture-based method was developed for screening of enzyme inhibitors from complex plant extracts directly. By elimination of false positives, three non-competitive α-glucosidase inhibitors were fished out from 400 μL green tea extract rapidly, using affinity capture of immobilized α-glucosidase combined with UHPLC-QTOF MS analysis.