Abstract
Breast cancer, affecting women mostly, is the second common cancer in the world. Previous studies showed that miRNAs played a role in the regulation of signal transduction pathways in many different stages of cancer. The SNPs that occur on miRNA can have broad impact in cancer by many different ways. It was found that SNP rs11614913 C>T in miR-196a2 related to breast cancer risk in Asia. In addition, the association between SNP rs6977848 T> G in miR-148a and breast cancer have early proven on the Australian. In this study, a combination of Tetra-Arms-PCR and PCR product melting analysis,was used to screen for the presence of the allele of rs11614913 and rs6977848 in Vietnamese breast cancer patients. The results showed that the frequency of C and T allele ofrs11614913 were 58.96 % and 41.04 % respectively; the frequency of T and G allele of rs6977848 were 99 % and 1 %, respectively. Based on the genotype frequencies in the HapMap, rs11614913 is predicted to be associated with breast cancer while rs6977848 is probably not related to breast cancer in Vietnam due to a highly dominant frequency of only one type allele. However, there is still need for further research on a larger sample size to confirm the association between the two SNPs with breast cancer in Vietnam.
Highlights
Previous studies showed that miRNAs played a role in the regulation of signal transduction pathways in many different stages of cancer
The SNPs that occur on miRNA can have broad impact in cancer by many different ways
It was found that SNP rs11614913 C>T in miR-196a2 related to breast cancer risk in Asia
Summary
Ung thư vú là loại ung thư phổ biến thứ hiện diện của các alen của hai hai trên thế giới và là ung thư thường gặp SNPrs11614913 và rs6977848 trên bệnh nhất ở phụ nữ. Mối quan hệ bệnh ung thư vú trong khi rs6977848 có lẽ giữa SNP rs6977848 T>G trên miR-148a và không liên quan đến ung thư vú ở Việt Nam ung thư vú đã có những kết quả ban đầu do chỉ có 1 kiểu alen phổ biến. Phương vẫn cần có nghiên cứu tiếp theo trên số pháp TAPMA (Tetra-Arms-PCR Melting lượng mẫu lớn hơn để khẳng định lại mối Analysis) là một phương pháp tầm soát kiểu liên quan giữa hai SNPs này với ung thư vú gen mới với sự kết hợp giữa T-Arms-PCR và ở Việt Nam để từ đó có thể chọn ra chỉ thị đọc kết quả bằng phân tích Tm các sản dùng trong chẩn đoán. Nghiên cứu này thực hiện khảo sát sự Từ khóa: Ung thư vú, miRNA, miR-196a2, miR-148a, SNP, rs11614913, rs6977848, TAPMA
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