Screening key micro RNAs for castration-resistant prostate cancer based on micro RNA activity analysis method

Abstract

Objective To explore the efficiency of micro RNA(miRNA) activity analysis method in screening the miRNA associated with castration resistance in prostate cancer. Methods miRNAs were screened for their potential role in castration resistance of prostate cancer by miRNAs activity analysis method. Human hormone-sensitive prostate cancer LNCAP cells (control group) and castration-resistant prostate cancer C4-2 cells (C4-2 group), PC-3 cells (PC-3 group) and DU-145 cells (DU-145 group) were cultured. Total RNA was extracted from each group. Real-time fluorescence quantitative PCR (qPCR) was used to detect miRNAs and compare the expression of miRNAs in each group. Results According to the screening process, 9 differentially expressed miRNAs were identified by miRNA activity analysis method. They were miR-1, miR-122, miR-218, miR-145, miR-155, miR-210, miR-197, miR-346 and let-7b. The 9 miRNAs were detected by qPCR. The results showed that 7 miRNAs were differentially expressed in prostate cancer cells in two different states. In different castration-resistant prostate cancer cells, miR-210, miR-197, miR-346 and miR-218 were significantly over expressed, while miR-122, miR-145 and let-7b were significantly under expressed. Conclusions The screening of castration-resistant transformation-related miRNAs in prostate cancer by miRNAs activity analysis method has high accuracy and reliability, and the specific transformation process needs further confirmation. Key words: Prostate cancer; Systemic biology; Micro RNA