Screening, Isolation and Biochemical Characterization of Novel Lipase Producing Bacteria from Soil Samples

Abstract

Novel Lipase producing bacterial isolates were obtained from the soil samples collected from three distinct and distant locations in India. The soil samples were found to have the microbial content with a range of (2.1-2.4) x105 CFU/gm; with direct proportionality between the lipase producing isolates and the microbial content in the soil samples. Lipase producing isolates were screened by tributyrin agar plates with 0.1% Rhodamine B dye and further screened to ob- tain the best possible lipase producing isolate(s) subjected to variable temperature and incubation period. The isolate B1 produced the lipase with maximum activity of 14 U/ml at 30℃ after 48hrs of incubation period at 150 rpm. Isolate B3 pro- duced the lipase at 37℃ consistently for five days with an average activity of 8.0 U/ml and this isolate may further be ex- plored for industrial production of lipase as part of a dietary supplement for fat and oil metabolism. Although the isolate B1 lacked consistency in its lipase productivity, it may further be studied to retain the higher activity and produce the lipase consistently with the use of different substrates and optimized parameters.