Screening, Identification and Production of L-asparagine from Lactobacillus reuteri of Healthy Mother Milk

Abstract

Human breast milk (HBM) may benefit Lactobacillus reuteri, a probiotic strain isolated from a mother's breast milk. It has been shown to inhibit pathogen growth, modulate the immune system, and have strong anti-inflammatory effects. This study aimed to isolate and screen Lactobacillus reuteri for L-asparagine production. Lactobacillus reuteri were grown in submerged fermentation and liquid media and used for secondary screening by the Nesslerization method. The isolates were identified based on morphological, cultural, and biochemical tests and API 50 to identify Lactobacillus reuteri. According to the findings, L-asparaginase isolated from bacteria showed positive L-asparaginase activity on a modified medium using phenol red as an indicator dye. However, bacterial isolates showed the highest enzyme activity, exhibiting a pink zone of hydrolysis of around 7.0 cm after 48h. and 4.92 IU/mg of specific activity. These results illustrated the importance of these isolates to produce and control L-asparagine for biomedical production in the future. Keywords: Lactobacillus reuteri, L-asparagine, L-asparaginase