Abstract
Antibodies can be used as probes to isolate directly a particular gene of interest. A recombinant DNA library is first constructed in an Escherichia coli expression vector, and foreign antigens are expressed in E. coli from the DNA inserts. The antigens produced are transferred onto nitrocellulose filters and then probed with antibody to detect the desired recombinant. This approach is rapid, and is particularly useful for isolating genes that are expressed at a low level and therefore are difficult to isolate using techniques employing nucleic acid probes (see Ref. 1) or by hybrid/selection methods.2 This chapter describes methods for isolating eukaryotic genes by constructing and screening E. coli expression vector libraries with antibody probes using the bacteriophage expression vector λgt11.3,4
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
