Abstract
Aging is associated with increased prevalence of skeletal and cardiac muscle disorders, such as sarcopenia and cardiac infarction. In this study, we constructed a compendium of purified ginsenoside compounds from Panax ginseng C.A. Meyer, which is a traditional Korean medicinal plant used to treat for muscle weakness. Skeletal muscle progenitor cell-based screening identified three compounds that enhance cell viability, of which 20(R)-ginsenoside Rh2 showed the most robust response. 20(R)-ginsenoside Rh2 increased viability in myoblasts and cardiomyocytes, but not fibroblasts or disease-related cells. The cellular mechanism was identified as downregulation of cyclin-dependent kinase inhibitor 1B (p27Kip1) via upregulation of Akt1/PKB phosphorylation at serine 473, with the orientation of the 20 carbon epimer being crucially important for biological activity. In zebrafish and mammalian models, 20(R)-ginsenoside Rh2 enhanced muscle cell proliferation and accelerated recovery from degeneration. Thus, we have identified 20(R)-ginsenoside Rh2 as a p27Kip1 inhibitor that may be developed as a natural therapeutic for muscle degeneration.
Highlights
There is a long history of the use of Panax ginseng C.A
A library of 39 purified ginsenosides was screened in skeletal muscle progenitor cells to detect compounds that increased cell proliferation, which is an indicator of the potential to enhance muscle regeneration12. 20(R)-ginsenoside Rh2 (CPP531) was identified as an enhancer of myoblast proliferation
A compendium of 39 ginsenosides purified from P. ginseng was screened in C2C12 murine myoblasts (Fig. 1A)
Summary
There is a long history of the use of Panax ginseng C.A. Meyer (P. ginseng) in Korean traditional medicine to treat reduced strength and cardiovascular disorders[1,2,3]. Meyer (P. ginseng) in Korean traditional medicine to treat reduced strength and cardiovascular disorders[1,2,3] This medicinal plant was considered to be of high value in East Asia and has been associated with numerous therapeutic effects on the human body[3]. A library of 39 purified ginsenosides was screened in skeletal muscle progenitor cells (myoblasts) to detect compounds that increased cell proliferation, which is an indicator of the potential to enhance muscle regeneration. CPP531 treatment in vitro increased Akt1/PKB activation and repressed expression of cyclin-dependent kinase inhibitor 1B (p27Kip[1])
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
