Screening for pigment production and characterization of pigment profile and photostability in cold-adapted Antarctic bacteria using FT-Raman spectroscopy

Abstract

Microbial pigments can replace synthetic organic pigments which often produced in unsustainable way and can be toxic. Therefore, search for new pigment producing microorganisms is of high interest for industry. In this study, a screening and characterization of pigment profile and photostability in seventy-four newly isolated Antarctic bacteria using Fourier-transform (FT) Raman spectroscopy and HPLC-MS was performed. Screening of the bacterial biomass by FT-Raman identified thirty-seven bacterial strains from the genera Agrococcus, Arthrobacter, Brachybacterium, Cryobacterium, Leifsonia, Micrococcus, Paeniglutamicibacter, Rhodococcus, Salinibacterium and Flavobacterium as having relatively high pigment content. The impact of growth temperature on the pigment production in the studied Antarctic bacteria was species - specific, while blue light exposure triggered pigment production in majority of the studied bacteria. HPLC-MS analysis of a biomass of a set of ten pigmented Antarctic bacteria identified eighteen different carotenoids and precursors. FT-Raman spectroscopy showed to be suitable for both, semi-qualitative library-independent identification of pigment producing bacteria and determination of pigment profile using spectral library of reference pigment standards. This study provides valuable insights into the pigment production capabilities of Antarctic bacteria and highlighting the potential of FT-Raman spectroscopy for characterizing microbial pigments.