Screening for low Raffinose family oligosaccharides and low Phytic acid lines in macro mutant Urdbean (Vigna mungoL. Hepper)

Abstract

Oligosaccharides are important component of urdbean in terms of metabolizable energy for monogastric animals and human. Sucrose, raffinose, stachyose and verbasecose are the four main oligosaccharides present in urdbean. Out of the four, only sucrose is nutritionally useful. When raffinose stachyose and verbascose are fermented by microbes present in the gut, the results are flatulence and discomfort, which ultimately lead to poor weight gain. The objectives of this work were to establish to identify low raffinose family oligosaccharide (RFO) and Low phytic acid (LPA) in macro mutants of TNAUCo (Bg) 6. The seeds of TNAUCo (Bg) 6 were treated with gamma rays (200Gy and 250Gy) EMS (15mM and 20mM). The frequency and spectrum of macro mutants different dose/concentration, number of chlorophyll mutants and Macro mutants observed. Chlorophyll mutants and viable mutants calculated based on the biological damage. The spectrum of chlorophyll mutants (Chlorina, Xantha, Albino and Viridis) and viable mutants (Tall and erect, dwarf, spreading, compact and bushy, crinkled and leathery, small leaf, big and broad leaf, narrow leaf, sterile, non flowering, pod and seed mutants) were observed in both generation. The spectrum of chlorophyll mutants increased with the decreased in dose of mutagen and increased with lower concentration. Total of 22 and 102 macro mutants were isolated from gamma ray and EMS treatments. Compare to all treatment 15mM of EMS treatments were establish more efficient in causing less biological injure and inducing highest amount of mutations. The total of 124 macro mutant three Low Verbascose mutant M2-F58 (0.06 mg/g), M2-F117 (0.13 mg/g) of 15mM, M2-F27 of 20mM (0.13 mg/g) and one low Phytate mutant in M2 -F37 of 15mM EMS (0.02 mg/g) were selected.