Screening for HPV infection in exfoliated cervical cells of women from different ethnic groups in Yili, Xinjiang, China

Zhenzhen Pan,Hongtao Li,Dongdong Cao,Weibin Wang,Dongmei Li,Junling Zhu,Yuning Song,Weinan Zheng,Xiangyi Zhe,Renfu Shao,Zemin Pan

doi:10.1038/s41598-019-39790-2

Abstract

We investigated the infection status and genotype distribution of human papillomavirus (HPV) in women of different ages and various ethnic groups in the Yili region, Xinjiang, China. We checked the HPV genotypes of 3,445 samples of exfoliated cervical cells using the PCR–reverse dot blot method. The total infection rate of HPV was 25.60% (882/3,445). The ethnic stratification showed that the infection rates were 22.87% (196/857) in Uygur, 21.55% (122/566) in Kazak, and 27.89% (564/2,022) in Han individuals. The most prevalent high-risk genotypes were HPV16, HPV52, and HPV53 in Uygur and Kazak and HPV16, HPV52, and HPV58 in Han ethnic groups. The age stratification showed that the infection rates in Han, Uygur, and Kazak women were up to 40.9% (61/149) in those aged 26–30 years, 41.5% (22/53) in those over 61 years old, and 30.2% (29/96) in those 46–50 years old, respectively. Therefore, HPV infection and HPV genotype distribution varied among the different age groups of the three ethnic groups.

Highlights

527,600 new cervical cancer cases and 265,700 cases of death due to cervical cancer were recorded worldwide in 2012
In 2015, the guidelines set by the Society of Gynecologic Oncology (SGO) and the American Society for Colposcopy and Cervical Pathology (ASCCP) addressed the issue of using HR-human papillomavirus (HPV) testing alone as the primary screening method, and recommended genotyping for HPV16 and 18 as a classification method for HR-HPV-positive women[9]
The HPV infection rate was significantly different among the three ethnic groups (χ2 = 13.797, P = 0.001) (Table 1)

Summary

Introduction

527,600 new cervical cancer cases and 265,700 cases of death due to cervical cancer were recorded worldwide in 2012. The incidence and mortality rates of cervical cancer in low-income countries are higher than those in high-income countries, and approximately 90% of cervical cancer deaths occur in developing countries[1]. More than 20 genotypes are classified as high-risk HPV, of which HPV16 and HPV18 are the most carcinogenic[4] Together, these two genotypes cause ~70% of cervical cancer cases. In 2015, the guidelines set by the Society of Gynecologic Oncology (SGO) and the American Society for Colposcopy and Cervical Pathology (ASCCP) addressed the issue of using HR-HPV testing alone as the primary screening method, and recommended genotyping for HPV16 and 18 as a classification method for HR-HPV-positive women[9]. We aimed to investigate the distribution of HPV genotypes in different ethnic and age groups in Yili, Xinjiang, China.

Objectives

Methods

Results

Conclusion