Abstract
ObjectiveThis research aims to explore differentially expressed circRNA between OA and KBD and potential diagnostic biomarkers. MethodsTotal RNA was extracted from 5 pairs of KBD and OA knee joint cartilage specimens, and the expression of circRNAs was analyzed by Chip Scanning Analysis. The microarray data was verified by quantitative polymerase chain reaction (qRT-PCR). CircRNA-miRNA network was constructed to predict targeting microRNAs of circRNA genes. Peripheral blood samples from 25 KBD patients and 25 OA patients were collected for verification by qRT-PCR. Diagnostic value was evaluated by the area under the receiver operator characteristic (ROC) curve. ResultsA total of 1627 circRNAs were differentially expressed between OA and KBD. Five bone and joint disease-related circRNAs were chosen for qRT-PCR validation. The difference in expression profile of hsa_circRNA_0020014 was confirmed by qRT-PCR, and its circRNA-miRNA regulation network was set up. The ROC curve demonstrated that hsa_circ_0020014_CBC1 in peripheral blood could distinguish patients with KBD and OA. ConclusionThe expression profiles of circRNA were significantly different between OA and KBD. hsa_circRNA_0020014 is a potential biomarker for differential diagnosis between these two diseases.
Highlights
CircRNAs belong to the non-coding RNA family and are highly expressed in eukaryotic cells
Our results suggested that the expression profiles of circRNA were significantly different between OA and Kashin–Beck disease (KBD). hsa_circRNA_0020014 is a potential biomarker for the differential diagnosis between these two diseases
It has been demonstrated that the circRNA transcripts of sex-determining region Y (SRY) and circular RNA CDR1as [4] can be used as miRNA sponges [5]
Summary
CircRNAs belong to the non-coding RNA family and are highly expressed in eukaryotic cells. Studies have shown that circRNA is widely involved in gene expression regulation, and has been proved to play an important role in the occurrence and development of nervous system diseases [6]. Due to their high degree of conservation [7], stablility [8] and accessible characteristics, the circRNAs could serve as biomarkers for diagnostics of clinical disease, such as gastric cancer [9], hepatocellular carcinoma [10] and oesophageal cancer [11]. The hsa_circ_0005836 was found as a biomarker and therapeutic target of active pulmonary tuberculosis [12]
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