Abstract

Iris germanica L. is an ornamental and medicinal plant used since ancient times for their rhizomes, still utilized today to obtain orris butter highly valued in perfumery. Iris germanica presents special root adaptations, which confers certain tolerance to water and salt stress, making it a good option in the context of the current climate trend. Aim of this study was to prospect the potential for biofortification of rhizomes using commercial arbuscular mycorrhizae (AM) application in field conditions for six Iris germanica cultivars. Plants presented Paris-type AM colonization. Rhizome samples collected after nine months from treatment and maturated, presented FT-IR (fourier transform infrared spectroscopy) spectra variation between experimental variants. Presence of the main metabolites in rhizome could be confirmed based on literature. Screening focused on two rhizome quality markers: carbohydrates, which influence plant development, and fatty acids, which are extractable from rhizome. Results suggest potential to enhance their accumulation in certain cultivars, such as ‘Pinafore Pink’ following AM application.

Highlights

  • Iris germanica L. is a widespread ornamental plant [1] and has been used since ancient times in Europe for their rhizomes, currently highly valued in perfumery

  • Rhizome samples collected after nine months from treatment and maturated, presented Fourier transform infrared spectroscopy (FT-IR) spectra variation between experimental variants

  • Root arbuscular mycorrhizae (AM) Colonization In June 2017 and after 8 months from planting in the field, AM colonization was observed for both sets of plants: inoculated and not inoculated

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Summary

Introduction

Iris germanica L. is a widespread ornamental plant [1] and has been used since ancient times in Europe for their rhizomes, currently highly valued in perfumery. Dry rhizome pieces are used as a spice, while rhizome extracts find application in the cosmetic industry and food industry as flavoring agents or in the production of alcoholic beverages such as gin [2]. Florentina and Iris pallida Lam [3,4] but almost any old garden bearded iris can be processed for this purpose with varying results [5]. Rhizomes harvested are either decorticated and slowly dried according to traditional method up to several years or sliced and subjected to biotechnological process for a fast-induced maturation [2,5,6]. “aged” orris is milled and steam distillated to obtain orris butter rich in irone and myristic acid [3,4,7,8] sold at 14640€ for 1 kg [9]

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